In vivo pharmacokinetic studies and intracellular delivery of methotrexate by means of glycine-tethered PLGA-based polymeric micelles

Publication date: 15 March 2017 Source:International Journal of Pharmaceutics, Volume 519, Issues 1–2 Author(s): Madhwi, Rajendra Kumar, Pramod Kumar, Bhupinder Singh, Gajanand Sharma, Om Prakash Katare, Kaisar Raza Methotrexate (MTX) is a widely used drug for the management of various kinds of cancers. However, numerous challenges are associated with MTX like poor aqueous solubility, dose-dependent side effects and poor-bioavailability. With an aim to explore the potential benefits in drug delivery of MTX, it was intended to fabricate glycine-PLGA-based polymeric micelles. Glycine was chemically linked to PLGA and the linkage was confirmed by FT-IR, and NMR-Spectroscopy. The developed polymeric micelles offered substantial loading to MTX with a pH-dependent drug release profile. The drug was released maximally at the cancer cell pH vis-à-vis blood plasma pH. The cytotoxicity of drug against MDA-MB-231 cell lines was enhanced by approx. 100% and the confocal laser scanning microscopy confirmed the localization of dye-tagged nanocarriers in the interiors of cancer cells. The bioavailable fraction of the drug was increased by approx. 4-folds, whereas elimination half-life was enhanced by around two-folds in Wistar rats. The novel approach offers a biodegradable and promising carrier for the better delivery of anticancer agents with immense promises of efficacy enhancement, improved delivery and better pharmacokinetic profile. Graphical abstract
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research

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Image courtesy of MARS Bioimaging New Zealand scientists have performed the first-ever 3-D, color X-ray on a human, using technology that could improve medical diagnostics in oncology, cardiology, neurology and orthopedics. Based on traditional black-and-white X-ray technology, the scanner incorporates the Medipix3RX detector chip, a particle-tracking technology developed for the CERN Large Hadron Collider. It was developed by the Medipix3 Collaboration, which comprises CERN in Geneva and 18 research institutions worldwide. The scanner records the energy of each photon as it collides with pixels while the shutter...
Source: Mass Device - Category: Medical Devices Authors: Tags: Blog Cardiovascular Diagnostics Imaging Neurological Orthopedics Research & Development CERN MARS Bioimaging Source Type: news
DISCUSSION: Although BCC is the most common skin cancer, it is usually a local disease and treated with local measures. Metastatic BCC is extremely rare, and in cases when surgical resection or local radiation are not viable options, chemotherapeutic agents typically offer very limited improvement. Vismodegib is an oral selective sonic hedgehog pathway inhibitor that shows benefit in patients with locally advanced or metastatic disease. PMID: 30005721 [PubMed - in process]
Source: The Permanente journal - Category: General Medicine Tags: Perm J Source Type: research
This study was not designed to explain whether supplementing with vitamin D will protect against asbestosis. This new research also does not examine if taking vitamin D will slow down the progression of existing ILD. Still, there are a lot of good reasons to pay attention to the sunshine vitamin. Regardless of whether a person has been exposed to asbestos, no one should ignore very low vitamin D levels. People already diagnosed with an asbestos-related cancer, may benefit from a quick check of vitamin D levels. According to the medical literature, up to 80 percent of people with cancer may be vitamin D deficient. People ca...
Source: Asbestos and Mesothelioma News - Category: Environmental Health Authors: Source Type: news
Molecular PharmaceuticsDOI: 10.1021/acs.molpharmaceut.8b00392
Source: Molecular Pharmaceutics - Category: Drugs & Pharmacology Authors: Source Type: research
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Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
Cancers, Vol. 10, Pages 234: Morphomechanical Alterations Induced by Transforming Growth Factor-β1 in Epithelial Breast Cancer Cells Cancers doi: 10.3390/cancers10070234 Authors: Mariafrancesca Cascione Valeria De Matteis Chiara C. Toma Stefano Leporatti The Epithelial to mesenchymal transition (EMT) is the process that drives epithelial tumor cells to acquire an invasive phenotype. The role of transforming growth factor-β1 (TGF-β1) in EMT is still debated. We used confocal laser scanning microscopy and scanning force spectroscopy to perform a morphomechanical analysis on epithelial bre...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research
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Source: Annals of Nuclear Medicine - Category: Nuclear Medicine Source Type: research
AbstractObjective2-Fluorine-18-fluoro-2-deoxy-d-glucose positron emission tomography (18F-FDG PET) imaging is not considered useful for assessing bladder cancer due to the physiological uptake of18F-FDG in the bladder. Despite reports of the detection of bladder cancer by washing out18F-FDG from the bladder, such methods are invasive and impractical in the routine practice. The purpose of this study was to evaluate prospectively the utility of oral hydration with 500  mL of water and voiding-refilling, a minimally invasive method that we introduced to enable detection of residual invasive bladder cancer on delayed18F-...
Source: Annals of Nuclear Medicine - Category: Nuclear Medicine Source Type: research
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Source: Hellenic Journal of Nuclear Medicine - Category: Nuclear Medicine Tags: Hell J Nucl Med Source Type: research
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Source: Hellenic Journal of Nuclear Medicine - Category: Nuclear Medicine Tags: Hell J Nucl Med Source Type: research
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