Docosahexaenoic acid loaded lipid nanoparticles with bactericidal activity against Helicobacter pylori

Publication date: 15 March 2017 Source:International Journal of Pharmaceutics, Volume 519, Issues 1–2 Author(s): Catarina Leal Seabra, Cláudia Nunes, Maria Gomez-Lazaro, Marta Correia, José Carlos Machado, Inês C. Gonçalves, Celso A. Reis, Salette Reis, M. Cristina L. Martins Docosahexaenoic acid (DHA), an omega-3 polyunsaturated fatty acid present in fish oil, has been described as a promising molecule to the treatment of Helicobacter pylori gastric infection. However, due to its highly unsaturated structure, DHA can be easily oxidized loosing part of its bioactivity. This work aims the nanoencapsulation of DHA to improve its bactericidal efficacy against H. pylori. DHA was loaded into nanostructured lipid carriers (NLC) produced by hot homogenization and ultrasonication using a blend of lipids (Precirol ATO5®, Miglyol-812®) and a surfactant (Tween 60®). Homogeneous NLC with 302±14nm diameter, −28±3mV surface charge (dynamic and electrophoretic light scattering) and containing 66±7% DHA (UV/VIS spectroscopy) were successfully produced. Bacterial growth curves, performed over 24h in the presence of different DHA concentrations (free or loaded into NLC), demonstrated that nanoencapsulation enhanced DHA bactericidal effect, since DHA-loaded NLC were able to inhibit H. pylori growth in a much lower concentrations (25μM) than free DHA (>100μM). Bioimaging studies, using scanning and transmission ele...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research

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Conclusions: Vietnamese patients with EOGC had higher rate of tumor extending to entire stomach compared to the older group. But the family history of GC, H. pylori infection and the pathological characteristics were not significantly different between the two groups. Environmental factors which play important roles in the development of EOGC in Vietnam should be investigated in future study. PMID: 30049355 [PubMed - in process]
Source: Asian Pacific Journal of Cancer Prevention - Category: Cancer & Oncology Tags: Asian Pac J Cancer Prev Source Type: research
Abstract A depressed lesion was found at a gastric angle of 76-year-old Japanese woman by esophagogastroduodenoscopy. Four years prior, she was diagnosed with a Helicobacter pylori infection but no eradication was performed. The pathological diagnosis of biopsy specimens was signet-ring cell carcinoma. Endoscopic submucosal dissection (ESD) was performed. Histopathological examination of the ESD specimen revealed proliferation of well-differentiated tubular adenocarcinoma mimicking fundic gland cells at the deep layer of the lamina propria mucosae. These tumor cells expressed focally pepsinogen-I, diffusely MUC6, ...
Source: World Journal of Gastroenterology : WJG - Category: Gastroenterology Authors: Tags: World J Gastroenterol Source Type: research
Conclusion: This study suggests no association between JCPyV infection and GC or other gastroduodenal diseases. The very low frequency of JCPyV LTag sequences in GC is an important aspect that weakens the hypothesis of the pathogenic role of JCPyV in gastric tumor induction.Gastrointest Tumors
Source: Gastrointestinal Tumors - Category: Gastroenterology Source Type: research
The objective of the present study was to evaluate the antioxidative, anti-inflammatory and anti-adhesive effect of I. chinensis on Helicobacter pylori (H. pylori)-induced gastric adenocarcinoma in an AGS cell model. Three types of I. chinensis extract were used: boiling water extract (BWE), ethanol extract (EE) and chloroform extract (CE) to check anti-H. pylori activity on the AGS cell line. Phytochemical and HPLC analysis were performed. In vitro antioxidant activity, including TEAC, DPPH and H2O2 scavenging abilities, was evaluated. In addition, the minimum inhibitory concentration (MIC) and minimum bactericidal concen...
Source: Journal of Herbal Medicine - Category: Complementary Medicine Source Type: research
Diagnosis of Helicobacter pylori infection : A short review. Wien Klin Wochenschr. 2018 Jun 29;: Authors: Tonkic A, Vukovic J, Vrebalov Cindro P, Pesutic Pisac V, Tonkic M Abstract Helicobacter pylori infections represent an important factor in the pathogenesis of chronic gastritis, peptic ulcer, MALT lymphoma and gastric adenocarcinoma. The recently published Maastricht V/Florence consensus report indicated that the urea breath test using 13 C urea still remains the best non-invasive test to diagnose H. pylori infections with high sensitivity and specificity. Among the stool antigen tests...
Source: Wiener Klinische Wochenschrift - Category: General Medicine Authors: Tags: Wien Klin Wochenschr Source Type: research
Introduction: H. pylori is proved to be strongly associated with development of gastric cancer. The aim of our study is to check H. pylori in newly diagnosed patients with gastric malignancy at different parts of the stomach and to define a correlation of it with H. pylori infection.
Source: Annals of Oncology - Category: Cancer & Oncology Source Type: research
In this study, authors investigated the clinical course of gastric MALT lymphoma and the pathogenic background in the view point of Correa ’s hypothesis.
Source: Annals of Oncology - Category: Cancer & Oncology Source Type: research
Conclusion: ME-NBI with VSCS can identify the DL in most patients (88.2%) with differentiated-type early gastric cancer afterHp eradication.Digestion 2018;98:175 –184
Source: Digestion - Category: Gastroenterology Source Type: research
Authors: Aljerian K Abstract Laparoscopic sleeve gastrectomy is a bariatric surgical procedure performed in patients with morbid obesity that provides the opportunity to review histopathological changes. The aim of this study was to characterize resected gastric specimens obtained from a Saudi patient population at a single center for a prospectively maintained database of laparoscopic sleeve gastrectomy cases. The histopathological findings from all patients undergoing laparoscopic sleeve gastrectomies at King Khalid University Hospital between 2010 and 2015 were retrospectively reviewed. Of the 602 cases reviewed...
Source: Gastroenterology Research and Practice - Category: Gastroenterology Tags: Gastroenterol Res Pract Source Type: research
In conclusion, for the first time, we report on the isolation of several scFvs against conserved residues of VacA toxin with high affinity and specificity, which may be used as novel diagnostic/therapeutic tool in the H. pylori infection. PMID: 29862446 [PubMed - as supplied by publisher]
Source: Applied Microbiology and Biotechnology - Category: Microbiology Authors: Tags: Appl Microbiol Biotechnol Source Type: research
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