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Docosahexaenoic acid loaded lipid nanoparticles with bactericidal activity against Helicobacter pylori

Publication date: 15 March 2017 Source:International Journal of Pharmaceutics, Volume 519, Issues 1–2 Author(s): Catarina Leal Seabra, Cláudia Nunes, Maria Gomez-Lazaro, Marta Correia, José Carlos Machado, Inês C. Gonçalves, Celso A. Reis, Salette Reis, M. Cristina L. Martins Docosahexaenoic acid (DHA), an omega-3 polyunsaturated fatty acid present in fish oil, has been described as a promising molecule to the treatment of Helicobacter pylori gastric infection. However, due to its highly unsaturated structure, DHA can be easily oxidized loosing part of its bioactivity. This work aims the nanoencapsulation of DHA to improve its bactericidal efficacy against H. pylori. DHA was loaded into nanostructured lipid carriers (NLC) produced by hot homogenization and ultrasonication using a blend of lipids (Precirol ATO5®, Miglyol-812®) and a surfactant (Tween 60®). Homogeneous NLC with 302±14nm diameter, −28±3mV surface charge (dynamic and electrophoretic light scattering) and containing 66±7% DHA (UV/VIS spectroscopy) were successfully produced. Bacterial growth curves, performed over 24h in the presence of different DHA concentrations (free or loaded into NLC), demonstrated that nanoencapsulation enhanced DHA bactericidal effect, since DHA-loaded NLC were able to inhibit H. pylori growth in a much lower concentrations (25μM) than free DHA (>100μM). Bioimaging studies, using scanning and transmission ele...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research

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Authors: Kino H, Nakano M, Kanamori A, Suzuki T, Kaneko Y, Tsuchida C, Tsuchida K, Tominaga K, Sasai T, Yamagishi H, Imai Y, Hiraishi H Abstract A 78-year-old man underwent endoscopic submucosal dissection (ESD) for early gastric adenocarcinoma twice in 2009 and 2014. Between the procedures, he successfully completed Helicobacter pylori eradication therapy. In May 2015, upper endoscopy screening showed two elevated lesions on the gastric fundus, and en bloc resection by ESD was performed. We histopathologically diagnosed the patient to have gastric adenocarcinoma of the fundic gland type. In this case, the two lesi...
Source: Internal Medicine - Category: Internal Medicine Tags: Intern Med Source Type: research
A 73-year-old man with a history of eradication of Helicobacter pylori and gastroduodenal ulcer 20 years previously was referred to our clinic for follow-up EGD. An elevated lesion with brownish pigmentation, 4  mm in diameter, was observed in the fundus of the stomach. Dilatated blood vessels were observed on the surface of the lesion, and there was no margin with the surrounding mucosa, as with a submucosal tumor (SMT) (A). Although the SMT-like shape and vasodilatation on the tumor surface were both ch aracteristic, brownish pigmentation is also characteristic of gastric adenocarcinoma of the fundic gland (chief ce...
Source: Gastrointestinal Endoscopy - Category: Gastroenterology Authors: Tags: At the focal point Source Type: research
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Source: Cancer Biomarkers - Category: Cancer & Oncology Tags: Cancer Biomark Source Type: research
o LF, Rendón-Huerta EP Abstract Gastric carcinogenesis has been associated to H. pylori virulence factors that induce a chronic inflammation process. Lipopolysaccharides play a role in chronic inflammatory responses via TLR2- and TLR4-dependent signaling pathways. Similarly, cellular invasiveness, metastatic potential and prognosis are usually associated to claudin-4, -6, -7 and -9 expression in gastric carcinogenesis. Therefore, the aim of this study was to determine if H. pylori LPS exerts an influence on carcinogenesis-related claudin expression and if it was directly regulated through the TLR2 pathway. ...
Source: Immunobiology - Category: Allergy & Immunology Authors: Tags: Immunobiology Source Type: research
Conclusion: Jordan is a low-risk area for gastric cancer, but carcinoma occurs at a young age and is associated with gastritis, Helicobacter pylori infection, and intestinal metaplasia in a large proportion of cases. Better strategic health planning and early detection is needed, especially in young patients suffering from gastritis. PMID: 28889157 [PubMed - in process]
Source: Saudi Medical Journal - Category: Middle East Health Tags: Saudi Med J Source Type: research
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Source: Journal of Cancer - Category: Cancer & Oncology Authors: Tags: Research Paper Source Type: research
Conclusion The anti-adhesion properties of the tested phytotherapeutic dietary ingredients were varied with HopQ genotypes. HopQ type 1 was found to be more sensitive to extracts of C. annum, C. longa and A. esculentus compared to the HopQ type 2 genotype.
Source: Journal of Integrative Medicine - Category: Complementary Medicine Source Type: research
Question: A 67-year-old man with a past medical history of well-controlled diabetes mellitus type 2, a recent episode of acute kidney injury owing to bilateral distal ureteral strictures of unknown etiology treated with bilateral ureteral stenting and percutaneous nephrostomy tube placement, and a remote history of adenocarcinoma of the gastric cardia, presented with a 27-pound weight loss, worsening lower abdominal pain, and change in stool for 2 weeks. Two years before the current presentation, he had been diagnosed with invasive adenocarcinoma (Figure A) of the gastric cardia (Seifert type 3) arising in a background of ...
Source: Gastroenterology - Category: Gastroenterology Authors: Tags: Electronic Clinical Challenges and Images in GI Source Type: research
The objective of the present study was to evaluate the antioxidative, anti-inflammatory and anti-adhesive effect of I. chinensis on Helicobacter pylori (H. pylori)-induced gastric adenocarcinoma in an AGS cell model. Three types of I. chinensis extract were used: boiling water extract (BWE), ethanol extract (EE) and chloroform extract (CE) to check anti-H. pylori activity on the AGS cell line. Phytochemical and HPLC analysis were performed. In vitro antioxidant activity, including TEAC, DPPH and H2O2 scavenging abilities, was evaluated. In addition, the minimum inhibitory concentration (MIC) and minimum bactericidal concen...
Source: Journal of Herbal Medicine - Category: Complementary Medicine Source Type: research
Abstract Helicobacter pylori (H. pylori) infection remains to be the major cause of important upper gastrointestinal diseases such as chronic gastritis, peptic ulcer, gastric adenocarcinoma and mucosa‐associated lymphoid tissue (MALT) lymphoma. H. pylori management in ASEAN: the Bangkok consensus report gathered key opinion leaders for the region to review and evaluate clinical aspects of H. pylori infection and to develop consensus statements, rationales and grades of recommendation for the management of H. pylori infection in clinical practice in ASEAN countries. This ASEAN Consensus consisted of 34 international exper...
Source: Journal of Gastroenterology and Hepatology - Category: Gastroenterology Authors: Tags: Solicited Review Article Source Type: research
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