Molecules, Vol. 22, Pages 86: Novel PEI/Poly-γ-Gutamic Acid Nanoparticles for High Efficient siRNA and Plasmid DNA Co-Delivery

In this study, we created a very high efficient DNA/siRNA co-delivery system by incorporating a negatively-charged poly- γ-glutamic acid (γ-PGA) into PEI/nucleic acid complexes. Spherical nanoparticles with about 200 nm diameter were formed by mixing PEI/plasmid DNA/siRNA/γ-PGA (dual delivery nanoparticles; DDNPs) with specific ratio (N/P/C ratio) and the particles present positive surface charge under all manufact uring conditions. The gel retardation assay shows both nucleic acids were effectively condensed by PEI, even at low N/P ratios. The PEI-based DDNPs reveal excellent DNA/siRNA transfection efficiency in the human hepatoma cell line (Hep 3B) by simultaneously providing high transgene expression effici ency and high siRNA silencing effect. The results indicated that DDNP can be an effective tool for gene therapy against hepatoma.

Source: Molecules - January 3, 2017 Category: Chemistry Authors: Shu-Fen Peng Hung-Kun Hsu Chun-Cheng Lin Ya-Ming Cheng Kuang-Hsing Hsu Tags: Article Source Type: research