Atorvastatin protected from Paraquat-induced cytotoxicity in Alveolar macrophages via down-regulation of TLR-4

Publication date: Available online 18 November 2016 Source:Environmental Toxicology and Pharmacology Author(s): Nazli Alizadeh-Tabrizi, Hassan Malekinejad, Soheil Varasteh, Hadi Cheraghi The current study designed to clarify the mechanism of paraquat-induced cytotoxicity and protective effects of Atorvastatin on freshly isolated alveolar macrophages (AMs). AMs were collected via bronchoalveolar lavage and exposed to various concentrations of paraquat in the presence and absence of atorvastatin for 24h. Cell viability, myeloperoxidase activity; nitric oxide generation and total antioxidant capacity were assessed. Expression of TLR-4 at mRNA and protein levels were studied by using PCR and western blot methods Atorvastatin enhanced the paraquat-reduced cell viability and reduced the paraquat-induced myeloperoxidase activity and nitric oxide production. Moreover, atorvastatin down-regulated by 60% the paraquat up-regulated expression of TLR-4 at protein and mRNA level. Our results suggest that, AMs in vitro model could be a novel cytological tool for studies on paraquat poisoning and therapy regimens. Additionally, atorvastatin cytoprotective effects on paraquat-induced cytotoxicity partly attribute to its anti-myeloperoxidase, antioxidant properties, which might be regulated via TLR-4 expression.
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research

Related Links:

We examined 9293 individuals from the Copenhagen General Population Study using nuclear magnetic resonance spectroscopy measurements of total cholesterol, free- and esterified cholesterol, triglycerides, phospholipids, and particle concentration. Fourteen subclasses of decreasing size and their lipid constituents were analysed: six subclasses were very low-density lipoprotein (VLDL), one intermediate-density lipoprotein (IDL), three low-density lipoprotein (LDL), and four subclasses were high-density lipoprotein (HDL). Remnant lipoproteins were VLDL and IDL combined. Mean nonfasting cholesterol concentration was 72â...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Conclusion: Our findings describe the most reported risk factors for preventability of oral anticoagulant-induced bleedings. These factors may be useful for targeting interventions to improve pharmacovigilance activities in our regional territory and to reduce the burden of medication errors and inappropriate prescription. Introduction Oral anticoagulant therapy is widely used for the prevention of stroke and systemic embolism in patients with atrial fibrillation, or for the prevention and treatment of deep vein thrombosis and pulmonary embolism (Raj et al., 1994; Monaco et al., 2017). Oral anticoagulants can be di...
Source: Frontiers in Pharmacology - Category: Drugs & Pharmacology Source Type: research
Joseph D. Romano1,2,3,4 and Nicholas P. Tatonetti1,2,3,4* 1Department of Biomedical Informatics, Columbia University, New York, NY, United States 2Department of Systems Biology, Columbia University, New York, NY, United States 3Department of Medicine, Columbia University, New York, NY, United States 4Data Science Institute, Columbia University, New York, NY, United States The discovery of new pharmaceutical drugs is one of the preeminent tasks—scientifically, economically, and socially—in biomedical research. Advances in informatics and computational biology have increased productivity at many stag...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
Fight Aging! provides a weekly digest of news and commentary for thousands of subscribers interested in the latest longevity science: progress towards the medical control of aging in order to prevent age-related frailty, suffering, and disease, as well as improvements in the present understanding of what works and what doesn't work when it comes to extending healthy life. Expect to see summaries of recent advances in medical research, news from the scientific community, advocacy and fundraising initiatives to help speed work on the repair and reversal of aging, links to online resources, and much more. This content is...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
In conclusion, ATS can attenuate PQ-induced pulmonary fibrosis. The mechanism may involve the downregulation of the HIF-1α/β‑catenin pathway and the inhibition of the PQ-induced EMT by ATS. ATS may be considered as a therapeutic agent for PQ poisoning-induced pulmonary fibrosis.
Source: Life Sciences - Category: Biology Source Type: research
We have to talk about drugs. No, not illicit drugs, but medications used by doctors and patients. Plaintiff attorneys run ads on TV that fool people into thinking certain meds are bad. The current one I deal with is the clot-blocking drug rivaroxaban (Xarelto.) Before that, it was dabigatran (Pradaxa). If, or when, the makers of rivaroxaban settle a class action suit, you can bet apixaban (Eliquis) will be next. These ads are a problem because they use fear persuasion (see Scott Adams’ blog), and because they can induce patients to stop taking or not starting a beneficial medicine.  Lots of other medications suf...
Source: Dr John M - Category: Cardiology Authors: Source Type: blogs
Looking back on my childhood, there was never really a time I wasn’t unsure of myself. I never thought I was cute enough, smart enough, funny enough or fun enough. In fact, I doubted that any of my playmates actually liked me. On my birthday, I wondered whether my friends would actually show up to my party. And if they did, was it because my parents paid them to come? If so, how much? How much was I worth? Decades later, I realize this was one of the first indications that I suffered from anxiety. Through countless hours of therapy, research and reflection, I have come to understand the many manifestations of anxiet...
Source: World of Psychology - Category: Psychiatry & Psychology Authors: Tags: Anxiety and Panic Medications Personal Psychotherapy Self-Esteem Cognitive Behavioral Therapy Hypertension overwhelm Panic Attack Paranoia Psychology self-worth Suffering Worry Source Type: blogs
Effects of Toxins I’ve been warning you about the toxins in our environment for years. They cause inflammation… They make you gain weight… They cause you to feel fatigued… Our exposure to them starts before we’re even born. One study found 287 chemicals and toxins in the blood of newborn babies.1 It’s shocking. What is in the air we breathe? We breathe in toxins and air pollutants every day. Carbon dioxide, lead, nitrogen oxide, sulfur dioxide, and particulate matter (PM). Causes of Stroke Now, a new study reveals that air pollution is responsible for as many as one-third of all strok...
Source: Al Sears, MD Natural Remedies - Category: Complementary Medicine Authors: Tags: Health Source Type: news
This study teaches us that poor wound healing and wrinkling and sagging that occur in aging skin share similar mechanisms." Reduced cell cohesiveness of outgrowths from eccrine sweat glands delays wound closure in elderly skin Human skin heals more slowly in aged vs. young adults, but the mechanism for this delay is unclear. In humans, eccrine sweat glands (ESGs) and hair follicles underlying wounds generate cohesive keratinocyte outgrowths that expand to form the new epidermis. Our results confirm that the outgrowth of cells from ESGs is a major feature of repair in young skin. Strikingly, in aged s...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
(NaturalNews) New science reveals that statin drugs DOUBLE the risk of diabetes. This is in addition to the extreme harm they cause to the body in other ways, including:• Accelerating aging.• Causing chronic muscle fatigue.• Compromising cognitive function.&bull...
Source: NaturalNews.com - Category: Consumer Health News Source Type: news
More News: Atorvastatin Calcium | Cytology | Drugs & Pharmacology | Environmental Health | Lipitor | Poisoning | Statin Therapy | Study | Toxicology | Zivast