Copper metabolism domain-containing 1 represses the mediators involved in the terminal effector pathways of human labour and delivery

STUDY HYPOTHESIS Does Copper Metabolism MURR1 Domain 1 (COMMD1) play a role in regulating the mediators involved in the terminal processes of human labour and delivery? STUDY FINDING COMMD1 plays a critical role in the termination of nuclear factor-B (NF-B) activity and the control of pro-inflammatory and pro-labour mediators. WHAT IS KNOWN ALREADY Inflammation and infection are the biggest aetiological factors associated with preterm birth. NF-B drives the transcription of pro-inflammatory mediators involved in the terminal effector pathways of human labour and delivery. In non-gestational tissues, COMMD1 is a negative regulator of NF-B-induced inflammation. STUDY DESIGN, SAMPLES/MATERIALS, METHODS The mRNA and/or protein level of COMMD1 was assessed in myometrium (n = 8 per group) and fetal membranes (n = 8 per group) obtained from term non-labouring and labouring women at term, and fetal membranes (n = 8 per group) at preterm with and without histological chorioamnionitis. Primary human myometrial cells were used to determine the effect of pro-inflammatory mediators on COMMD1 level, and the effect of COMMD1 small interfering RNA (siRNA) on pro-labour mediators. Statistical significance was ascribed to a P < 0.05. MAIN RESULTS AND THE ROLE OF CHANCE COMMD1 expression was significantly decreased with spontaneous term labour in myometrium; in fetal membranes with histologically confirmed chorioamnionitis and in myometrial cells treated with pro-inflammatory cytokines i...
Source: Molecular Human Reproduction - Category: Molecular Biology Authors: Tags: Articles Source Type: research