Abstract A66: Comprehensive gene expression analysis of ductal carcinoma in situ (DCIS) progression to invasive breast cancer reveals potential biomarkers

The big challenge in breast cancer is to discriminate which patients with non-invasive disease would benefit from minimal clinical interventions from those who require a more traditional treatment approach, keeping the highest survival rates. Hence, identification of molecular alterations of epithelial cells that take place in earliest stages of ductal carcinoma in situ (DCIS) progression is crucial for understanding the invasion process and for guiding the design of intervention strategies. In order to assess the molecular changes that occur during progression of non-invasive breast cancer we analyzed gene expression of epithelial cells representative of two steps of DCIS progression - before and after invasion manifestation. Thus, for assessing the molecular alterations before invasion manifestation, epithelial cells were captured by laser microdissection from in situ component of both groups of lesions - pure DCIS and in situ component that co-exist with invasive breast carcinoma lesion (DCIS-IBC), and for alterations after invasion manifestation, epithelial cells were captured from in situ and invasive components of the same DCIS-IBC lesion. Their transcriptional profiles were interrogated by three different approaches (cDNA microarray, Rapid Subtraction Hybridization (RaSH) cDNA library and RT-qPCR assay) and gene expression profiles were assessed under a comprehensive manner for detecting differences in gene expression level that may occur in the two steps of progressio...
Source: Molecular Cancer Research - Category: Cancer & Oncology Authors: Tags: Other Topics: Poster Presentations - Proffered Abstracts Source Type: research