Abstract 3320: Identification of potential molecular targets related to radioresistance in human oral cancers

In India, oral cancer is the most common cancer in males and ranks third among females, attributed predominantly to the use of smokeless tobacco. Radiotherapy is an integral part of oral cancer treatment either alone or in combination with surgery and chemotherapy. However the development of radioresistance creates a hurdle in the efficacy of radiotherapy. Therefore, exploring the differential molecular profile of established radioresistant versus parental oral cancer cells may help in predicting the clinical effectiveness of radiotherapy.The present study aims to profile the radioresistant cell lines established by low dose radiation from their parental oral cancer cell lines, to identify the molecules and the possible mechanisms associated with radioresistance in oral cancer.We have established three radioresistant oral cancer cell lines i.e. 70Gy-AW13516, 70Gy-AW8507 and 70Gy-SCC029B derived from their parental AW13516, AW8507 and SCC029B cell lines respectively using in-vitro fractionated ionizing radiation (FIR). A clinically admissible 2Gy radiation dose was given using a 60Co-γ linear accelerator (Bhabhatron-2, ACTREC) upto 70Gy totally for each of the cell lines. The radioresistant character was determined by clonogenic cell survival assay and proteomic profiling of parental versus radioresistant cells was done by two-dimensional gel electrophoresis. Gels were analysed by PD-Quest software (Bio-Rad) and differential spot identities were revealed by MALDI-TOF/TOF (Bru...
Source: Cancer Research - Category: Cancer & Oncology Authors: Tags: Tumor Biology Source Type: research