Insights into the differential proteome landscape of a newly isolated Paramecium multimicronucleatum in response to cadmium stress

J Proteomics. 2024 Apr 16:105178. doi: 10.1016/j.jprot.2024.105178. Online ahead of print.ABSTRACTEmploying microbial systems for the bioremediation of contaminated waters represent a potential option, however, limited understanding of the underlying mechanisms hampers the implication of microbial-mediated bioremediation. The omics tools offer a promising approach to explore the molecular basis of the bioremediation process. Here, a mass spectrometry-based quantitative proteome profiling approach was conducted to explore the differential protein levels in cadmium-treated Paramecium multimicronucleatum. The Proteome Discoverer software was used for the identification and quantification of the differentially abundant proteins. The proteome profiling generated 7416 peptide spectral matches, yielding 2824 total peptides, corresponding to 989 proteins. The analysis revealed 29 proteins exhibited significant (p ≤ 0.05) differential levels, including higher abundance of 6 proteins and reduced levels of 23 proteins in Cd2+ treated samples. These differentially abundant proteins were found to be associated with stress response, energy metabolism, protein degradation, cell growth, and hormone processing. Briefly, a comprehensive proteome profile in response to cadmium stress of a newly isolated Paramecium has been established that will be useful in future studies identifying critical proteins involved in the bioremediation of metals in ciliates. SIGNIFICANCE: Ciliates are considered ...
Source: Journal of Proteomics - Category: Biochemistry Authors: Source Type: research