GSE231529 SARS-COV-2 putative mi-RNAs may target host immune system and cellular mechanisms to mediate pathogenesis-an in silico and in vivo study II

Contributors : Mohit K Rai ; Sukanya Tripathy ; Anamika K Anuja ; Durga P Misra ; Rupali Awale ; Latika Gupta ; Pankti Mehta ; Gaurav Pande ; Supriya Sharma ; Zia Hashim ; Alok Nath ; Mohan Gurjar ; Afzal Azeem ; Naveen R_ ; Sundaram Gopalakrishnan ; Thomas N Koshy ; Neha Singh ; Vishwesh Agarwal ; Ajmal Khan ; Vikas AgarwalSeries Type : Expression profiling by RT-PCROrganism : Homo sapiensThe exact role of epigenetics in the pathogenesis of severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) is unclear. Herein we have hypothesized that viral micro RNAs (miRNAs) may modulate host cellular and immune responses which may lead to varied clinical presentations. Using bioinformatics tools, we have first predicted putative miRNAs in the genotype of the SARS-CoV-2 and their potential gene targets. Thereafter, quantification of mRNA of key genes regulating inflammation, coagulation, neuropsychiatric functions, fibrosis and glucose metabolism was evaluated in the peripheral blood mononuclear cells and bone marrow of patients suffering from different severities of SARS-CoV-2 infection. Key genes regulating innate immunity, lymphopoiesis, T-cell and B-cell development, cytokine storm, coagulation pathways, fibrosis, neuropsychiatric manifestations, glucose metabolism, and olfaction were significantly downregulated in severe infection when compared with mild infection. Downregulated genes mRNA recovered to normal/near normal levels on recovery from infection. SA...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by RT-PCR Homo sapiens Source Type: research