Directed Differentiation of Neurons from Human iPSCs for Modeling Neurological Disorders

Methods Mol Biol. 2024;2794:141-155. doi: 10.1007/978-1-0716-3810-1_12.ABSTRACTHuman-induced pluripotent stem cell (hiPSC) technology has enabled comprehensive human cell-based disease modeling in vitro. Due to limited accessibility of primary human neurons as well as species-specific divergence between human and rodent brain tissues, hiPSC-derived neurons have become a popular tool for studying neuronal biology in a dish. Here, we provide methods for transcription factor-driven directed differentiation of neurons from hiPSCs via a neural progenitor cell (NPC) intermediate. Doxycycline-inducible expression of neuron fate-determining transcription factors neurogenin 2 (NGN2) and achaete-scute homolog 1 (ASCL1) enables rapid and controllable differentiation of human neurons for disease modeling applications. The provided method is also designed to improve the reproducibility of human neuron differentiation by reducing the batch-to-batch variation of NPC differentiation and lentiviral transduction.PMID:38630226 | DOI:10.1007/978-1-0716-3810-1_12
Source: Mol Biol Cell - Category: Molecular Biology Authors: Source Type: research