Viruses, Vol. 16, Pages 623: ML241 Antagonizes ERK 1/2 Activation and Inhibits Rotavirus Proliferation

In this study, a new anti-rotavirus compound was selected by ELISA, and cell activity was detected from 453 small-molecule compounds. The anti-RV effects and underlying mechanisms of the screened compounds were explored. In vitro experimental results showed that the small-molecule compound ML241 has a good effect on inhibiting rotavirus proliferation and has low cytotoxicity during the virus adsorption, cell entry, and replication stages. In addition to its in vitro effects, ML241 also exerted anti-RV effects in a suckling mouse model. Transcriptome sequencing was performed after adding ML241 to cells infected with RV. The results showed that ML241 inhibited the phosphorylation of ERK1/2 in the MAPK signaling pathway, thereby inhibiting IκBα, activating the NF-κB signaling pathway, and playing an anti-RV role. These results provide an experimental basis for specific anti-RV small-molecule compounds or compound combinations, which is beneficial for the development of anti-RV drugs.

Source: Viruses - April 17, 2024 Category: Virology Authors: Jinlan Wang Xiaoqing Hu Jinyuan Wu Xiaochen Lin Rong Chen Chenxing Lu Xiaopeng Song Qingmei Leng Yan Li Xiangjing Kuang Jinmei Li Lida Yao Xianqiong Tang Jun Ye Guangming Zhang Maosheng Sun Yan Zhou Hongjun Li Tags: Article Source Type: research