Inhibition of Crif1 protects fatty acid-induced POMC neuron damage by increasing CPT-1 function

Am J Physiol Endocrinol Metab. 2024 Apr 10. doi: 10.1152/ajpendo.00420.2023. Online ahead of print.ABSTRACTHypothalamic proopiomelanocortin neurons are sensors of signals that reflect the energy stores in the body. Inducing mild stress in proopiomelanocortin neurons protect them from the damage promoted by the consumption of a high-fat diet, mitigating the development of obesity; however, the cellular mechanisms behind these effects are unknown. Here, we induced mild stress in a proopiomelanocortin neuron cell line by inhibiting Crif1. In proopiomelanocortin neurons exposed to high levels of palmitate, the partial inhibition of Crif1 reverted the defects in mitochondrial respiration and ATP production; this was accompanied by improved mitochondrial fusion/fission cycling. Furthermore, the partial inhibition of Crif1 resulted in increased reactive oxygen species production, increased fatty acid oxidation, and reduced dependency on glucose for mitochondrial respiration. These changes were dependent on the activity of CPT-1. Thus, we identified a CPT-1-dependent metabolic shift towards greater utilization of fatty acids as substrates for respiration as the mechanism behind the protective effect of mild stress against palmitate-induced damage of proopiomelanocortin neurons.PMID:38597829 | DOI:10.1152/ajpendo.00420.2023

Source: American Journal of Physiology. Endocrinology and Metabolism - April 10, 2024 Category: Physiology Authors: Lara Regina-Ferreira Fernando Valdivieso-Rivera Monara K S C Angelim Larissa Menezes Dos Reis Vanessa O de Oliveira Furino Joseane Morari Lizandra Maia de Souza Silvio R Consoni Carlos H Sponton Pedro M Moraes Vieira Licio A Velloso Source Type: research