Sens-ocular model: Cell-based assay to evaluate eye stinging potential of chemicals and baby cosmetic formulations
In this study, HEK-293 cells with high TRPV1 expression were utilized to evaluate calcium influx related to receptor activation triggered by chemicals and cosmetic formulations. The cells were exposed to increasing concentrations of substances to cause or not some aggression to the eye, and TRPV1 activity was assessed by measuring intracellular FURA-2 AM fluorescence signal. To confirm TRPV1 channel activation, capsazepine, a capsaicin antagonist, was employed in addition to using capsaicin as a positive control. The study's results indicate that this novel model can identify compounds known to cause some aggression to the eye, such as stinging, considering a cut-off value of 60% of Ca2+ influx exposed to the lowest evaluated concentration (0.00032%). When applied to the cosmetic baby formulation, although the presented model exhibited higher sensitivity by classifying as stinging formulations that had previously undergone clinical testing and were deemed non-stinging, the assay could serve as a valuable in vitro tool for predicting human eye stinging sensation and can be used as a tier 1 in an integrated testing strategy.PMID:38614139 | DOI:10.1016/j.tiv.2024.105824
Source: Toxicology in Vitro - Category: Toxicology Authors: Lara Barroso Brito Carolina Motter Catarino Maria Claudia Passos Artur C Garcia Silva Desiree Cigaran Schuck Andrezza Di Pietro Micali Canavez Marize Campos Valadares Source Type: research