Decoding T Cell Clonal Dynamics in Acute Lung Rejection

Purpose: T cell-mediated acute cellular rejection (ACR) is a common complication in lung transplant (LTx) recipients within the first post-transplant year, affecting 30-50% of patients and is often associated with later development of chronic lung allograft dysfunction (CLAD). Traditional genomics studies, utilizing bulk RNA-Seq, lack the cellular granularity required to completely understand ACR. Our research leverages single-cell RNA +TCR sequencing (scRNAseq/TCRseq) to investigate the clonal repertoire and gene expression changes in expanded T cells during ACR, highlighting the crucial role of single-cell TCR sequencing in elucidating clonal dynamics and T-cell gene expression, and paving the way for potential therapeutic interventions.
Source: The Journal of Heart and Lung Transplantation - Category: Transplant Surgery Authors: Source Type: research