Strategies for Inducing and Validating Zinc Deficiency and Zinc Repletion

Am J Physiol Heart Circ Physiol. 2024 Apr 5. doi: 10.1152/ajpheart.00025.2024. Online ahead of print.ABSTRACTGiven the growing interest in the role of zinc in the onset and progression of diseases, there is a crucial demand for reliable methods to modulate zinc homeostasis. Using a dietary approach, we provide validated strategies to alter whole-body zinc in mice, applicable across species. For confirmation of zinc status, animal growth rates as well as plasma and urine zinc levels were evaluated. The accessible and cost-effective methodology outlined will increase scientific rigor, ensuring reproducibility in studies exploring the impact of zinc deficiency and repletion on the onset and progression of diseases.PMID:38578238 | DOI:10.1152/ajpheart.00025.2024
Source: American Journal of Physiology. Heart and Circulatory Physiology - Category: Physiology Authors: Source Type: research