BEST4 < sup > + < /sup > cells in the intestinal epithelium

Am J Physiol Cell Physiol. 2024 Apr 1. doi: 10.1152/ajpcell.00042.2024. Online ahead of print.ABSTRACTThe recent development of single cell transcriptomics highlighted the existence of a new lineage of mature absorptive cells in the human intestinal epithelium. This subpopulation is characterized by the specific expression of Bestrophin 4 (BEST4) and of other marker genes including OTOP2, CA7, GUCA2A, GUCA2B, and SPIB. BEST4+ cells appear early in development and are present in all regions of the small and large intestine at a low abundance (<5% of all epithelial cells). Location-specific gene expression profiles in BEST4+ cells suggest their functional specialization in each gut region, as exemplified by the small intestine-specific expression of the ion channel CFTR. The putative roles of BEST4+ cells include sensing and regulation of luminal pH, tuning of guanylyl cyclase-C signaling, transport of electrolytes, hydration of mucus and secretion of antimicrobial peptides. However, most of these hypotheses lack functional validation, notably because BEST4+ cells are absent in mice. The presence of BEST4+ cells in human intestinal organoids indicates that this in vitro model should be suitable to study their role. Recent studies showed that BEST4+ cells are also present in the intestinal epithelium of macaque, pig, zebrafish and, here, we report their presence in rabbit, which suggests that these species could be appropriate animal models to study BEST4+ cells during develo...
Source: Am J Physiol Cell Ph... - Category: Cytology Authors: Source Type: research