GSE225277 Balanced spatiotemporal arrangements of Histone H3 and H4 post-translational modifications are necessary for meiotic prophase I chromosome organization

Contributors : Lava K Surarapu ; Aradhana mohanthy ; anjali kumari ; ajith kumar ; ranjiht kumar ; mohd Athar ; R K Gandham ; H Prasada RaoSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusDynamic nuclear architecture and chromatin organizations are the key features of the mid- prophase I in mammalian meiosis. Where the chromatin undergoes major changes, including meiosis-specific spatiotemporal arrangements and remodelling, the establishment of chromatin loop –axis structure, pairing, and crossing over between homologous chromosomes, any deficiencies in these events may induce genome instability, subsequently leading to failure to produce gametes and infertility. Despite the significance of chromatin structure, little is known about the location of chr omatin marks and the necessity of their balance during meiosis prophase I. Here, we show a thorough cytological study of the surface-spread meiotic chromosomes of mouse spermatocytes for H3K9,14,18,23,27,36, H4K12,16 acetylation, and H3K4,9,27,36 methylation. Active acetylation and methylation marks on H3 and H4, such as H3K9ac, H3K14ac, H3K18ac, H3K36ac, H3K56ac, H4K12ac, H4K16ac, and H3K27me3 and H3K36me3 exhibited pan-nuclear localization away from heterochromatin. In comparison, repressive marks like H3K9me3 are localized to heterochromatin. In addition, we found that the intensities of H3 K23ac, H3K27ac, and H3K9me3 enhanced during meiotic prophase I. Further, taking advantage...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research