GSE243695 Plasma amino acids regulate pancreatic acinar cell proliferation and size via mTORC1 and YAP/TAZ signaling pathways

Contributors : Chunhua Dai ; Yulong Gong ; Yue Zhang ; Amber Bradley ; Zihan Tang ; Katelyn Sellick ; Shristi Shrestha ; Erick Spears ; Brittney A Covington ; Jade Stanley ; Regina Jenkins ; Tiffany M Richardson ; Rebekah A Brantley ; Katie Coate ; Diane C Saunders ; Jordan Wrights ; Marcela Brissova ; Danielle Dean ; Alvin C Powers ; Wenbiao ChenSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusPancreas volume or mass varies more than 3-fold among adult humans. The heterogeneity is likely the result of genetics, diseases, and nutrition. Dietary protein intake and blood amino acid levels are known to affect pancreas mass, but the underlying mechanism is not well understood. The goal of this study is to determine how increased blood amino acid level (hyperaminoacidemia) induces pancreas expansion.Multiple complementary mouse and zebrafish models were used to study the impact of hyperaminoacidemia on pancreatic mass, acinar cell size and proliferation. Blood amino acid levels were manipulated by dietary protein content, or by pharmacologic or genetic interruption of glucagon signaling (IGS). The activation of mammalian target of rapamycin complex 1 (mTORC1) and Yes-associated protein 1 (YAP) were determined by pS6 and YAP staining. Sirolimus administration in mice and knockdown of solute carrier family 38 member 5b (slc38a5b) and yap/taz in zebrafish were used to determine the role of mTORC1, SLC38A5 and YAP/TAZ in acinar cell...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research