An isothermal nucleic acid amplification-based enzymatic recombinase amplification method for dual detection of porcine epidemic diarrhea virus and porcine rotavirus A

In this study, a dual ERA method to detect and distinguish between PEDV and PoRVA nucleic acids was established. The method shows high sensitivity, as the detection limits were 101 copies/μL for both viruses. To test the usefulness of this method in clinical settings, we tested 64 swine clinical samples. Our results were 100% matched with those acquired using a commercially available kit. Therefore, we have successfully developed a dual diagnostic ERA nucleic acids method for detecting and distinguishing between PEDV and PoRVA.PMID:38522136 | DOI:10.1016/j.virol.2024.110062

Source: Virology - March 24, 2024 Category: Virology Authors: Qianwen Wu Xing Liu Jingyu Wang Sijia Xu Fanliang Zeng Ling Chen Guihong Zhang Heng Wang Source Type: research