Computer-aided exploration of multiobjective optimal temperature profiles in slow freezing for human induced pluripotent stem cells

This study was based on a model that calculates cell survival rates after thawing, and the model was extended to evaluate cell potentials until 24 h after seeding. To estimate parameter values for this extension, freezing experiments were performed using constant cooling rates. Using quality and productivity indicators, we evaluated 16,206 temperature profiles using our model, and a promising profile was obtained. Finally, an experimental investigation of the profile was undertaken, and the contribution of the temperature profile to both quality and productivity was confirmed.PMID:38513997 | DOI:10.1016/j.cryobiol.2024.104885
Source: Cryobiology - Category: Biology Authors: Source Type: research