Integrated network pharmacology and experimental verification to explore the potential mechanism of San Ying decoction for treating triple-negative breast cancer

In this study, the active ingredients of each herbal medicine composing SYF and their target proteins are obtained from the Traditional Chinese Medicine Systems Pharmacology database. Breast cancer-related genes are obtained from the GeneCards database. Major targets and pathways related to SYF treatment in breast cancer are identified by analyzing the above data. By conducting molecular docking analysis, we find that the active ingredients quercetin and luteolin bind well to the key targets KDR1, PPARG, SOD1, and VCAM1. In vitro experiments verify that SYF can reduce the proliferation, migration, and invasion ability of TNBC cells. Using a TNBC xenograft mouse model, we show that SYF could delay tumor growth and effectively inhibit the occurrence of breast cancer lung metastasis in vivo. PPARG, SOD1, KDR1, and VCAM1 are all regulated by SYF and may play important roles in SYF-mediated inhibition of TNBC recurrence and metastasis.PMID:38516703 | DOI:10.3724/abbs.2024015

Source: Acta Biochimica et Biophysica Sinica - March 22, 2024 Category: Biochemistry Authors: Xiaojuan Yang Feifei Li Youyang Shi Yuanyuan Wu Rui Yang Xiaofei Liu Yang Zhang Guangtao Zhang Mei Ma Zhanyang Luo Xianghui Han Ying Xie Sheng Liu Source Type: research