Molecules, Vol. 29, Pages 1449: Royal Jelly Enhances the Ability of Myoblast C2C12 Cells to Differentiate into Multilineage Cells

Molecules, Vol. 29, Pages 1449: Royal Jelly Enhances the Ability of Myoblast C2C12 Cells to Differentiate into Multilineage Cells Molecules doi: 10.3390/molecules29071449 Authors: Takumi Ito Thira Rojasawasthien Sachiko Yamashita Takeuchi Hideto Okamoto Nobuaki Okumura Tomohiko Shirakawa Takuma Matsubara Tatsuo Kawamoto Shoichiro Kokabu Royal jelly (RJ) is recognized as beneficial to mammalian health. Multilineage differentiation potential is an important property of mesenchymal stem cells (MSCs). C2C12 cells have an innate ability to differentiate into myogenic cells. Like MSCs, C2C12 cells can also differentiate into osteoblast- and adipocyte-lineage cells. We recently reported that RJ enhances the myogenic differentiation of C2C12 cells. However, the effect of RJ on osteoblast or adipocyte differentiation is still unknown. Here in this study, we have examined the effect of RJ on the osteoblast and adipocyte differentiation of C2C12 cells. Protease-treated RJ was used to reduce the adverse effects caused by RJ supplementation. To induce osteoblast or adipocyte differentiation, cells were treated with bone morphogenetic proteins (BMP) or peroxisome proliferator-activated receptor γ (PPARγ) agonist, respectively. RNA-seq was used to analyze the effect of RJ on gene expression. We found that RJ stimulates osteoblast and adipocyte differentiation. RJ regulated 279 genes. RJ treatment upregulated glutathione-related genes. Glut...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research