Simultaneous Dual ‐Gene Test of Methicillin‐Resistant Staphylococcus Aureus using an Argonaute‐Centered Portable and Visual Biosensor

The STAR platform is developed for simultaneous dual-gene test of methicillin-resistantStaphylococcus aureus using an Argonaute-centered portable biosensor. With the platform, the targeted nucleic acid signals gene are dexterously converted into fluorescent signals. A portable 3D-printed device that integrated functions of heating reaction and fluorescence detection is designed, showing the potential of PON for drug-resistant pathogenic bacteria and others. AbstractThe development of novel method for drug-resistant bacteria detection is imperative. A simultaneous dual-gene Test of methicillin-resistantStaphylococcus aureus (MRSA) is developed using an Argonaute-centered portable biosensor (STAR). This is the first report concerning Argonaute-based pathogenic bacteria detection. Simply, the species-specificmecA andnuc gene are isothermally amplified using loop-mediated isothermal amplification (LAMP) technique, followed by Argonaute-based detection enabled by its programmable, guided, sequence-specific recognition and cleavage. With the strategy, the targeted nucleic acid signals gene are dexterously converted into fluorescent signals. STAR is capable of detecting thenuc gene andmecA gene simultaneously in a single reaction. The limit of detection is 10 CFU/mL with a dynamic range from 10 to 107 CFU/mL. The sample-to-result time is<65  min. This method is successfully adapted to detect clinical samples, contaminated foods, and MRSA-infected animals. This work broadens the ...
Source: Small - Category: Nanotechnology Authors: Tags: Research Article Source Type: research