Influence of Zika virus on the cytotoxicity, cell adhesion, apoptosis and inflammatory markers of glioblastoma cells
Oncol Lett. 2024 Feb 28;27(4):176. doi: 10.3892/ol.2024.14309. eCollection 2024 Apr.ABSTRACTGlioblastoma (GBM) is one of the most common types of brain tumor in adults. Despite the availability of treatments for this disease, GBM remains one of the most lethal and difficult types of tumors to treat, and thus, a majority of patients die within 2 years of diagnosis. Infection with Zika virus (ZIKV) inhibits cell proliferation and induces apoptosis, particularly in developing neuronal cells, and thus could potentially be considered an alternative for GBM treatment. In the present study, two GBM cell lines (U-138 and U-251) were infected with ZIKV at different multiplicities of infection (0.1, 0.01 and 0.001), and cell viability, migration, adhesion, induction of apoptosis, interleukin levels and CD14/CD73 cell surface marker expression were analyzed. The present study demonstrated that ZIKV infection promoted loss of cell viability and increased apoptosis in U-138 cells, as measured by MTT and triplex assay, respectively. Changes in cell migration, as determined by wound healing assay, were not observed; however, the GBM cell lines exhibited an increase in cell adhesion when compared with non-tumoral cells (Vero). The Luminex immunoassay showed a significant increase in the expression levels of IL-4 specifically in U-251 cells (MOI 0.001) following exposure to ZIKV. There was no significant change in the expression levels of IFN-γ upon ZIKV infection in the cell lines tested. F...
Source: Oncology Letters - Category: Cancer & Oncology Authors: Daniel Rodrigo Marinowic Gabriele Goulart Zanirati Pamella Nunes Azevedo Ângela Zanatta Ismael Plentz Allan Marinho Alcar á Fernanda Bueno Morrone Thamiris Becker Scheffel Ang élica Regina Cappellari Paulo Michel Roehe Ana Paula Muterle Varela Denise C Source Type: research