Co-infection of influenza A viruses of swine contributes to effective shuffling of gene segments in a naturally reared pig.

Co-infection of influenza A viruses of swine contributes to effective shuffling of gene segments in a naturally reared pig.
Virology. 2015 Jun 23;484:203-212
Authors: Abe H, Mine J, Parchariyanon S, Takemae N, Boonpornprasert P, Ubonyaem N, Patcharasinghawut P, Nuansrichay B, Tanikawa T, Tsunekuni R, Saito T
Abstract
Following the 2009 H1N1 pandemic, surveillance activities have been accelerated globally to monitor the emergence of novel reassortant viruses. However, the mechanism by which influenza A viruses of swine (IAV-S) acquire novel gene constellations through reassortment events in natural settings remains poorly understood. To explore the mechanism, we collected 785 nasal swabs from pigs in a farm in Thailand from 2011 to 2014. H3N2, H3N1, H1N1 and H1N2 IAVs-S were isolated from a single co-infected sample by plaque purification and showed a high degree of diversity of the genome. In particular, the H1N1 isolates, possessing a novel gene constellation previously unreported in Thailand, exhibited greater variation in internal genes than H3N2 IAVs-S. A pair of isolates, designated H3N2-B and H1N1-D, was determined to have been initially introduced to the farm. These results demonstrate that numerous IAVs-S with various gene constellations can be created in a single co-infected pig via reassortment.
PMID: 26115167 [PubMed - as supplied by publisher]

Source: Virology - June 23, 2015 Category: Virology Authors: Abe H, Mine J, Parchariyanon S, Takemae N, Boonpornprasert P, Ubonyaem N, Patcharasinghawut P, Nuansrichay B, Tanikawa T, Tsunekuni R, Saito T Tags: Virology Source Type: research