In vitro evaluation of the susceptibility of bacterial biofilms to Hecogenin Acetate

Acta Trop. 2024 Feb 20:107157. doi: 10.1016/j.actatropica.2024.107157. Online ahead of print.ABSTRACTBiofilms are a bacterial resistance strategy through which microorganisms organize themselves in the form of a colony fixed to a surface that is protected by a polymer matrix. Infectious diseases that result in biofilm formation have been considered a relevant public health problem due to the potential to increase patient morbidity and mortality, in addition to increasing the burden on health services. Such pathologies are treated with the use of antimicrobial drugs, the indiscriminate use of which has contributed to the process of bacterial resistance, demanding the need to invest in new alternatives to combat them. Based on this, the present work aimed to evaluate the anti-biofilm formation and eradication capacity of Hecogenin Acetate, a steroidal sapogenin of natural origin with important antibacterial properties. For this, we used strains of Streptococcus mutans INCQS 00446 (ATCC 25175), Enterococcus faecalis INCQS 00018 (ATCC 14506), Staphylococcus epidermidis INCQS 00016 (ATCC 12228), Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 9027 and Escherichia coli ATCC 259223. The formation, formation inhibition and treatment assays were carried out in microdilution plates and revealed using the crystal violet method. Readings were carried out using absorbance at wavelengths of 492nm. All tests were performed in triplicate and statistical analyzes were performed ...
Source: Acta Tropica - Category: Infectious Diseases Authors: Source Type: research