The noncanonical nucleotide binding site 1 of the bile salt export pump is optimized for proper function of the transporter

In this study, we mutated two deviant residues in the signature sequence to generate a double mutant (R1221G/E1223Q). Furthermore, a triple mutant (E502S/R1221G/E1223Q) was generated, in which the deviant residues of the signature sequence and Q-loop were mutated concurrently to canonical residues. The double and triple mutants showed 80% and 60%, respectively, of the activity of wild-type BSEP. As expected, an increasing number of mutations gradually impair transport as an intricate network of interactions within the ABC proteins ensures proper functioning.PMID:38328902 | DOI:10.1002/cbin.12136
Source: Cell Biology International - Category: Cytology Authors: Source Type: research