Identification of dysregulation of sphingolipids in retinoblastoma using liquid chromatography-mass spectrometry

Exp Eye Res. 2024 Jan 19:109798. doi: 10.1016/j.exer.2024.109798. Online ahead of print.ABSTRACTRetinoblastoma (RB) is a rare ocular cancer seen in children that counts for approximately 3% of all childhood cancers. It is found that mutation in RB1, a tumour Suppressor Gene on chromosome 13 as the cause of malignancy. Retinoblastoma protein is the target for ceramide to cause apoptosis. We studied lipidomics of two RB cell lines, one aggressive cell line (NCC-RbC-51) derived from a metastatic site and one non aggressive cell line (WERI-Rb1) in comparison with a control cell line (MIO-M1). Lipid profiles of all the cell lines were studied using high resolution mass spectrometer coupled to high performance Liquid Chromatography. Data acquired from all the three cell lines in positive mode were analyzed to identify differentially expressed metabolites. Several phospholipids and lysophospholipids were found to be dysregulated. We observed upregulation of hexosyl ceramides, and down regulation of dihydroceramides and higher order sphingoglycolipids hinting at a hindered sphingolipid biosynthesis. The results obtained from Liquid Chromatography-Mass Spectrometry are validated by using qPCR and it was observed that genes involved in ceramide biosynthesis pathway are getting down regulated.PMID:38246332 | DOI:10.1016/j.exer.2024.109798

Source: Experimental Eye Research - January 21, 2024 Category: Opthalmology Authors: Omkar Surendra Khade Sruthy Sasidharan Ankit Jain Bhavani Shankar Maradani Amit Chatterjee Divya Gopal Ranjith Kumar Ravi Kumar Subramaniyan Krishnakumar Akhilesh Pandey Narayanan Janakiraman Sailaja V Elchuri Seetaramanjaneyulu Gundimeda Source Type: research