Multilocus genotyping for classification and genetic structuring of Lactobacillus casei: insights from source and geographical origin

AbstractThe aim of the study in this article is to systematise the newly introduced strains ofLactobacillus based on determining the nucleotide sequence of a particular set of their genes (loci). The primary approach employed to address this issue involves conducting a laboratory experiment. During this experiment, a thorough examination was carried out on a set of organic compounds consisting of small DNA elements from theLactobacillus genus. TheMultilocus genotyping method served as the central technique, complemented by additional molecular-biological and population methods. These additional methods were utilized to determine the extent of phylogenetic similarity among pure cultures ofLactobacillus and to classify them accordingly. The article presents the gene isolates that were used forMultilocus typing; the number ofL. casei isolates suitable forMultilocus genotyping was revealed; the gene alleles that allowed classifyingL. casei isolates into five sequencing types were revealed; the effectiveness of genetic typing method forMultilocus sequencing was substantiated. The article is of practical value for microbiologists and geneticists in the field of molecular biology, as well as for technologists in the food industry. With the development of applied methods in genetic systematics, it has become possible to study pure culture ofLactobacillus species. The application of modern methods of genotypic classification ofLactobacillus species will make it possible to increase th...
Source: World Journal of Microbiology and Biotechnology - Category: Microbiology Source Type: research