EXPLORING THE FLUCONAZOLE-RESISTANCE MODIFYING ACTIVITY AND POTENTIAL MECHANISM OF ACTION OF FIXED OIL FROM Caryocar coriaceum WITTM. (CARYOCARACEAE) AGAINST Candida SPECIES

This study aimed to evaluate the chemical composition, antifungal activity, reduction of fungal virulence, and toxicity of the fixed oil from the inner mesocarp of C. coriaceum tested against three Candida yeasts. The oil was characterized by gas chromatography (GC-MS and GC-FID). Antifungal activity was assessed using the serial microdilution method. Additionally, the potential of the oil as an enhancer of fluconazole action was tested at sub-inhibitory concentrations (MIC/8). The mechanisms of action of the fixed oil of C. coriaceum on the morphological transition of Candida spp. strains and their virulence factors. The chemical composition of the fixed oil of C. coriaceum comprised both unsaturated and saturated fatty acids. Oleic (61%) and palmitic (33%) acids were the major constituents. In terms of its anti-Candida activity, the oil reduced the growth of C. albicans (IC50: 371 µg/mL) and C. tropicalis (IC50: 830 µg/mL). Furthermore, the oil reversed the antibiotic resistance of C. albicans and C. tropicalis, redering them sensitive to fluconazole and reducing their IC50 from 12.33 µg/mL and 362 µg/mL to 0.22 µg/mL and 13.93 µg/mL, respectively. The observed antifungal activity may be attributed to the overproduction of reactive oxygen species.PMID:38196022 | DOI:10.1002/cbdv.202301960
Source: Chemistry and Biodiversity - Category: Biochemistry Authors: Source Type: research