Differences in vasomotor function of mesenteric arteries between Ossabaw minipigs with predisposition to metabolic syndrome and G öttingen minipigs

Am J Physiol Heart Circ Physiol. 2023 Dec 22. doi: 10.1152/ajpheart.00719.2023. Online ahead of print.ABSTRACTMetabolic syndrome predisposes and contributes to the development and progression of atherosclerosis. The minipig strain "Ossabaw" is characterized by a predisposition to develop metabolic syndrome. We compared vasomotor function in Ossabaw minipigs before they develop their diseased phenotype to that of Göttingen minipigs- without such genetic predisposition. Mesenteric arteries of adult Ossabaw and Göttingen minipigs were dissected post mortem and mounted on a myograph for isometric force measurements. Maximal vasoconstriction to potassium chloride (KClmax) was induced. Cumulative concentration-response curves were determined in response to norepinephrine. Endothelium-dependent (with carbachol) and -independent (with nitroprusside) vasodilation were analyzed after preconstriction by norepinephrine. In a bioinformatic analysis, variants/altered base pairs within genes associated with cardiovascular disease were analyzed. KClmax was similar between the minipig strains (15.6±6.7 vs. 14.1±3.4 ΔmN). Vasoconstriction in response to norepinephrine was more pronounced in Ossabaw than in Göttingen minipigs (increase of force to 143±48 vs. 108±38% of KClmax). Endothelium-dependent and -independent vasodilation were less pronounced in Ossabaw than in Göttingen minipigs (decrease of force to 46.4±29.6 vs. 16.0±18.4% and to 36.7±25.2 vs. 2.3±3.7% of norepinephrine-i...
Source: American Journal of Physiology. Heart and Circulatory Physiology - Category: Physiology Authors: Source Type: research