Antioxidant and Anti-Obesity Effects of < em > Juglans mandshurica < /em > in 3T3-L1 Cells and High-Fat Diet Obese Rats

J Microbiol Biotechnol. 2023 Dec 18;34(4):1-10. doi: 10.4014/jmb.2311.11032. Online ahead of print.ABSTRACTJuglans mandshurica Maxim. walnut (JMW) is well-known for the treatment of dermatosis, cancer, gastritis, diarrhea, and leukorrhea in Korea. However, the molecular mechanism underlying its anti-obesity activity remains unknown. In the current study, we aimed to determine whether JMW can influence adipogenesis in 3T3-L1 preadipocytes and high-fat diet rats and determine the antioxidant activity. The 20% ethanol extract of JMW (JMWE) had a total polyphenol content of 133.33±2.60 mg GAE/g. Considering the antioxidant capacity, the ABTS and DPPH values of 200 µg/mL of JMWE were 95.69±0.94 and 79.38±1.55%, respectively. To assess the anti-obesity activity of JMWE, we analyzed the cell viability, fat accumulation, and adipogenesis-related factors, including CCAAT-enhancer-binding protein alpha (C/EBPα), sterol regulatory element-binding protein-1c (SREBP1c), peroxisome proliferator-activated receptor-gamma (PPARγ), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC). We found that total lipid accumulation and triglyceride levels were reduced, and the fat accumulation rate decreased in a dose-dependent manner. Furthermore, JMWE suppressed adipogenesis-related factors C/EBPα, PPARγ, and SREBP1c, as well as FAS and ACC, both related to lipogenesis. Moreover, animal experiments revealed that JMWE could be employed to prevent and treat obesity-related diseases. Henc...
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Source Type: research