Astragalus polysaccharide promotes the regeneration of intestinal stem cells through HIF-1 signalling pathway

This study was aimed to investigate the potential effects of APS on IR-induced intestine injury via promoting the regeneration of ISCs. We have established models of IR-induced intestinal injury and our results showed that APS played great radioprotective effects on the intestine. APS improved the survival rate of irradiated mice, reversed the radiation damage of intestinal tissue, increased the survival rate of intestinal crypts, the number of ISCs and the expression of intestinal tight junction-related proteins after IR. Moreover, APS promoted the cell viability while inhibited the apoptosis of MODE-K. Through organoid experiments, we found that APS promoted the regeneration of ISCs. Remarkably, the results of network pharmacology, RNA sequencing and RT-PCR assays showed that APS significantly upregulated the HIF-1 signalling pathway, and HIF-1 inhibitor destroyed the radioprotection of APS. Our findings suggested that APS promotes the regeneration of ISCs through HIF-1 signalling pathway, and it may be an effective radioprotective agent for IR-induced intestinal injury.PMID:38098246 | DOI:10.1111/jcmm.18058

Source: J Cell Mol Med - December 15, 2023 Category: Molecular Biology Authors: Qianqian Ding Xianpeng Zu Wei Chen Jiayun Xin Xike Xu Yanhui Lv Xintong Wei Jie Wang Yanping Wei Zhanhong Li Jianming Cai Jicong Du Weidong Zhang Source Type: research