Development and evaluation of a multiplex digital PCR method for sensitive and accurate detection of respiratory pathogens in children

In this study, a multiplex dPCR method was developed and evaluated as a means of detecting five respiratory pathogens in children with acute lower respiratory tract infection (ALRTI). With 139 nasopharyngeal swabs collected from children with ALRTI, pathogens were detected using dPCR and quantitative real-time PCR (qPCR) methods. Of those specimens, dPCR detected 86 positive cases, while qPCR identified 84. Moreover, dPCR exhibited higher sensitivity than qPCR, and displayed no cross-reactivity with common respiratory pathogens. These findings suggest that dPCR-based method could become one of the most promising options for acute respiratory pathogen detection.PMID:38064870 | DOI:10.1016/j.virol.2023.109948

Source: Virology - December 8, 2023 Category: Virology Authors: Xiangpeng Chen Fang Wang Yiliang Fu Luci Huang Fei Li Hongwei Zhao Xiaolei Guan Qiuping Li Qi Li Yilu Wang Yong Guo Zhengde Xie Source Type: research