Inhibition of biofilm, quorum ‐sensing, and swarming motility in pathogenic bacteria by magnetite, manganese ferrite, and nickel ferrite nanoparticles

AbstractResistance to antibiotics by pathogenic bacteria constitutes a health burden and nanoparticles (NPs) are being developed as alternative and multipurpose antimicrobial substances. Magnetite (Fe3O4 np), manganese ferrite (MnFe2O4 np) and nickel ferrite (NiFe3O4 np) NPs were synthesized and characterized using thermogravimetric analysis, transmission electron microscopy, Fourier transformed infra-red, and X-ray diffraction. The minimal inhibitory concentrations (MIC) ranged from 0.625 to 10  mg/mL against gram-positive (Staphylococcus aureus ATCC 25923 andEnterococcus faecalis ATCC 29212), gram-negative (Escherichia coli ATCC 25922 andPseudomonas aeruginosa ATCC 27853) and candida (Candida albicans ATCC 10239 andCandida tropicalis ATCC 13803) species. The NPs exhibited violacein inhibition againstChromobacterium violaceum CV12472 of 100% at MIC and reduced to 27.2%  ± 0.8% for magnetite NPs, 12.7% ± 0.3% for manganese ferrite NPs and 43.1% ± 0.2% for nickel ferrite NPs at MIC/4. Quorum-sensing (QS) inhibition zones againstC. violaceum CV026 were 12.5  ±0.6 mm for Fe3O4 np, 09.1  ± 0.5 mm for MnFe3O4 NP and 17.0  ± 1.2 mm for NiFe3O4 np. The NPs inhibited swarming motility againstP. aeruginosa PA01 and biofilm against six pathogens and the gram-positive biofilms were more susceptible than the gram-negative ones. The NiFe2O4 np had highest antibiofilm activity against gram-positive and gram-negative bacteria as well as highest QS inhibition while Fe3O4...
Source: Biotechnology and Applied Biochemistry - Category: Biochemistry Authors: Tags: ORIGINAL ARTICLE Source Type: research