Comparison of in-gel and in-solution proteolysis in the proteome profiling of organ perfusion solutions

ConclusionsThis study showed that in-solution digestion is a more efficient method for LC-MS/MS analysis of kidney and liver organ perfusion solutions. This method is also quicker and easier than in-gel digestion, allowing for greater sample throughput, with fewer opportunities for experimental error or peptide loss.
Source: Clinical Proteomics - Category: Biochemistry Source Type: research