Natural terpene-based derivatives to control postharvest sclerotinia rot and the involved potential mechanism

Int J Food Microbiol. 2023 Oct 27;409:110461. doi: 10.1016/j.ijfoodmicro.2023.110461. Online ahead of print.ABSTRACTIn an effort to develop novel postharvest preservatives of satisfactory environmental compatibility from natural monoterpene, a series of terpene-based derivatives containing oxime ester were designed and prepared. In this research, the inhibitory effect of target compounds against S. sclerotiorum were evaluated though in vitro and in vivo tests. It was investigated that most compounds exhibited promising antifungal activity, especially compound 4k with EC50 value of 3.02 μg/mL, which was significantly superior to commercial fungicide trifloxystrobin. Notably, compound 4k improved the physicochemical quality of carrot including weight loss, contents of titratable acidity, ascorbic acid, carotenoid, malondialdehyde, and enzymes activities, ensuring post-harvest preservation. Simultaneously, the mycelial morphology, ultrastructure, cell wall permeability, and defense/respiration-related enzymes of S. sclerotiorum were destructed. The preliminary toxicity evaluation of target compounds indicated that the prepared target compounds possessed safety and low toxicity. Additionally, the essential chemical features for activity and interaction mode between molecule and cytochrome bc1 complex were inquired by computer-aid technology. The study provided meaningful insight into formulation of natural terpene-based fresh-keeping agent to resist postharvest decay infected by...
Source: International Journal of Food Microbiology - Category: Food Science Authors: Source Type: research