Recent Progress in Prompt Molecular Detection of Exosomes Using CRISPR/Cas and Microfluidic-Assisted Approaches Toward Smart Cancer Diagnosis and Analysis

ChemMedChem. 2023 Nov 2:e202300359. doi: 10.1002/cmdc.202300359. Online ahead of print.ABSTRACTNano exosomes are essential indicators of molecular mechanisms involved in interacting with cancer cells and the tumor environment. The nano exosomes, as nanostructures based on lipids and nucleic acids, provide a communication pathway for information transfer by transporting biomolecules from the target cell to other cells. Importantly, these extracellular vesicles are released into the blood flow by the most invading cells, i.e., cancer cells; thereby, they could be considered a promising specific biomarker for cancer diagnosis. The CRISPR-Cas systems, as a genome editing approach, provide the inactivation possibility of genes or even the complete removal of the gene from the cell without affecting intracellular mechanisms. These practical systems provide vital information about the involved factors in cancer development that could lead to more effective cancer treatment. Meanwhile, microfluidic approaches can also significantly benefit cancer research due to their proper sensitivity, high throughput, low material consumption, low cost, and advanced spatial and temporal control. Thereby, employing CRISPR-Cas- and microfluidic-based approaches toward nano exosome monitoring could be considered a valuable source of information for cancer therapy and diagnosis. This review assessed the recent progress in these promising diagnosis approaches toward accurate cancer therapy and in-depth...

Source: ChemMedChem - November 2, 2023 Category: Chemistry Authors: Seyyed Mojtaba Mousavi Seyyed Alireza Hashemi Masoomeh Yari Kalashgrani Vahid Rahmanian Mohsen Riazi Navid Omidifar Raed H Althomali Mohammed M Rahman Wei-Hung Chiang Ahmad Gholami Source Type: research