Respiratory exposure to agricultural dust extract promotes increased intestinal < em > Tnf α < /em > expression, gut barrier dysfunction, and endotoxemia in mice

We report that 3-week intranasal exposure of HDE is associated with increased intestinal macromolecule permeability and elevated serum endotoxin concentrations in C57BL/6J mice. In vivo studies indicated mislocalization of the epithelial cell adhesion protein, E-cadherin, in the colon as well as an increase in the pro-inflammatory cytokine, Tnfa, in the proximal colon. Moreover, mRNA expression of the Paneth cell associated marker, Lyz1was increased the proximal colon while the expression of the goblet cell marker, Muc2 was unchanged in the epithelial cells of the ileum, cecum and distal colon. These results demonstrate that airway exposure to CAFOs dusts promote airway inflammation and modify the gastrointestinal tract to increase intestinal permeability, induce systemic endotoxemia and promote intestinal inflammation. Therefore, this study identifies complex physiological consequences of chronic exposure to organic dusts derived from CAFOs on the gut-lung axis.PMID:37874654 | DOI:10.1152/ajpgi.00297.2022
Source: Am J Physiol Gastroi... - Category: Gastroenterology Authors: Source Type: research