Matrix metalloproteinase 2 degrades collagen I to regulate ovarian development by association with an insulin ‐like peptide

The ovary generally undergoes tissue remodeling during the larval to pupal transition, with membrane degenerating and ovarioles growing to ensure the maturity of the eggs in insects. In our study, we found that BmILP induces the phosphorylation of AKT at the S1 stage to regulate MMP2 expressing highly in the S2 stage, and it would have negative feedback for AKT dephosphorylation. At the PP stage, MMP2 degrades collagen I to promote cell proliferation, cell migration, and ECM remodeling, which results in ovarian capsule degradation. On the other hand, BmILP remained highly expressed to promote the development of ovarian and oocyte. WhileMMP2 knockout directly leads to collagen I accumulation, the imbalance of AKT expression andBmILP reduction results in ovarian capsule non-degradation, cell apoptosis, and ovarioles become inelastic and fibrotic, without the inability to provide space for oocyte development. AbstractThe ovary generally undergoes tissue remodeling during larval to pupal transition, which includes membrane degeneration and ovariole growth. At the same time, the hormones produced by insects significantly change during metamorphosis. However, the regulatory mechanism for ovarian development and hormones is not fully understood in insects. Herein, we found that matrix metalloproteinase 2 (MMP2) was highly expressed in the ovarian capsules and ovarioles, and the development was abnormal after knocking out MMP2 inBombyx mori. The process of abnormal degradation of col...

Source: Insect Science - October 17, 2023 Category: Biology Authors: Yi Wei, Xiao ‐Lin Zhou, Peng Chen, Tai‐Hang Liu, Cheng Lu, Min‐Hui Pan Tags: ORIGINAL ARTICLE Source Type: research