Investigation of LncRNA PVT1 and MiR-21-5p Expression as Promising Novel Biomarkers for Autism Spectrum Disorder

AbstractThe characteristics of ncRNA in children with autism spectrum disorder (ASD) were observed to disclose a theoretical basis for further research on molecular markers for early warning of ASD. Children with ASD and normal control children were recruited to collect peripheral blood RNA samples. The concentration ofPVT1 andmiR-21-5p was quantitatively analyzed by qRT-PCR. Pearson correlation coefficient method was used to evaluate the link betweenPVT1 level andmiR-21-5p level of the children. Receiver operating characteristic (ROC) curves were applied to reckon the predictive value ofPVT1,miR-21-5p, and their combination in ASD. The interconnection ofPVT1 withmiR-21-5p was represented by luciferase reporter assay. The targeted genes ofmiR-21-5p were predicted. The enrichment and protein interaction analysis of these genes was carried out to find the important core genes and analyze their value in ASD. In the disease group, the level ofPVT1 was downregulated, while the content ofmiR-21-5p was upregulated. The expression level of serummiR-21-5p was negatively correlated with the level ofPVT1. Luciferase reporter gene assay documented thatPVT1 directly targetedmiR-21-5p. ROC curve showed thatPVT1,miR-21-5p, and their combination showed clinical value for disease diagnosis. The functional enrichment analysis showed that the targets ofmiR-21-5p participated in ASD by regulating related functions and pathways. Reduced expression ofPVT1 and raisedmiR-21-5p were good diagnostic m...
Source: Journal of Molecular Neuroscience - Category: Neuroscience Source Type: research