N6-methyladenosine modification of PLOD2 causes spermatocyte damage in rats with varicocele

CONCLUSION: As a result, we found that varicocele-induced oxidative stress promoted PLOD2 expression level via m6A methylation modification. In addition, targeting m6A demethylation of PLOD2 by CRISPR/dCas13b-ALKBH5 system can regulate GC-2 cell proliferation and apoptosis under oxidative stress. Taken together, our study has acquired a better understanding of the mechanisms underlying male infertility associated with oxidative stress, as well as a novel therapeutic target for male infertility.PMID:37670228 | DOI:10.1186/s11658-023-00475-4
Source: Cellular and Molecular Biology Letters - Category: Biochemistry Authors: Source Type: research