Establishment of a TNFRSF11B knock-out human induced pluripotent stem cell line (KSCBi002-B-2) via CRISPR/Cas9 system

In this report, we generated a biallelic knock-out hiPSC line for the TNFRSF11B gene via CRISPR/Cas9. When TNFRSF11B Knock-out hiPSCs were differentiated into mesenchymal progenitor cells (MPCs), the expression level of OPG was significantly decreased compared to normal hiPSC-derived MPCs. This knock-out hiPSCs will provide a chance to study Paget disease of bone 5 (juvenile Paget disease).PMID:37481965 | DOI:10.1016/j.scr.2023.103167

Source: Cell Research - July 23, 2023 Category: Cytology Authors: Jujin Jeong Dahye Lee Byeong-Cheol Park Tae-Hoon Lee Sang-Wook Park Source Type: research