Properties of cell wall polysaccharides of raw nectarine fruits after treatment under conditions that modulate gastric digestion

Int J Biol Macromol. 2023 Jun 24:125460. doi: 10.1016/j.ijbiomac.2023.125460. Online ahead of print.ABSTRACTThe results of the study of the physicochemical properties of the high-molecular-weight soluble and insoluble components of nectarine cell walls obtained by fruit treatment under conditions that modulate of gastric digestion are presented. Homogenized nectarine fruits were sequentially treated by natural saliva and simulated gastric fluid (SGF) at pH 1.8 and 3.0. The isolated polysaccharides were compared with polysaccharides obtained by sequential extraction of nectarine fruit with cold, hot, and acidified water, solutions of ammonium oxalate and sodium carbonate. As a result, high-molecular-weight water-soluble pectic polysaccharides, weakly bound in the cell wall, were extracted into the gastric environment, regardless of pH. Homogalacturonan (HG) and rhamnogalacturonan-I (RG-I) were identified in all pectins. It was shown that their quantity and ability to form highly viscous solutions determine high values of the rheological characteristics of the nectarine mixture formed under simulated gastric conditions. The modifications occurring with the insoluble components under the influence of acidity of SGF were importance. They determined difference in the physicochemical properties of both the insoluble fibres and the nectarine mixtures.PMID:37364806 | DOI:10.1016/j.ijbiomac.2023.125460
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Source Type: research