Selenite bioreduction with concomitant green synthesis of selenium nanoparticles by a selenite resistant EPS and siderophore producing terrestrial bacterium

Biometals. 2023 Apr 29. doi: 10.1007/s10534-023-00503-y. Online ahead of print.ABSTRACTEnvironmental bacterial isolates play a very important role in bioremediation of metals and toxic metalloids. A bacterial strain with high selenite (SeO32-) tolerance and reducing capability was isolated from electronic waste dump site in Banaras Hindu University, Varanasi, India. Based on 16 S rRNA sequencing and BLAST search, this bacterial isolate was identified as Bacillus paramycoides and designated as strain MF-14. It tolerated Sodium selenite up to 110 mM when grown aerobically in LB broth and reduced selenite into elemental selenium (Se0) significantly within 24 h with concomitant biosynthesis of selenium nanoparticles as clearly revealed by brick red precipitate and specific surface plasmon resonance peak at 210 nm using UV-Visible spectrophotometer. Scanning electron microscopy (SEM) analysis of this bacterial strain exposed to 1mM and 5 mM selenite also demonstrated morphological alterations as cell enlargement due to accumulation and bioprecipitation of elemental selenium (Se0). The FTIR analysis clearly demonstrated that functional groups present on the surface of biogenic selenium nanoparticles (SeNPs) play a significant role in the stabilization and capping of SeNPs. Furthermore, these SeNPs were characterized using spectroscopic analysis involving Dynamic light scattering, zeta potential, XPS, FTIR, XRD and Raman spectroscopy which clearly revealed particle size 10-700 nm, a...
Source: Biometals - Category: Biochemistry Authors: Source Type: research