Intra-arterial administration of placenta-derived decidual stromal cells to the superior mesenteric artery in the rabbit: distribution of cells, feasibility and safety.

Intra-arterial administration of placenta-derived decidual stromal cells to the superior mesenteric artery in the rabbit: distribution of cells, feasibility and safety. Cell Transplant. 2015 May 13; Authors: Arnberg F, Lundberg J, Olsson A, Samén E, Jaff N, Jussing E, Dahlén U, Nava S, Axelsson R, Ringdén O, Kaipe H, Holmin S Abstract Selective administration of mesenchymal stromal cells to the mesenteric arteries is a potential technique to overcome pulmonary trapping and increase the density of transplanted cells in extensive mural inflammation of the intestine such as in inflammatory bowel disease and graft-versus-host disease. We injected 5 x 10(6) (111)In-oxine-labelled human decidual stromal cells (DSCs) to the rabbit superior mesenteric artery (SMA) using clinical routine catheters guided by an angiographical system under sterile conditions. We used longitudinal single photon emission tomography at 6-hours and at 1, 2 and 5 days to assess trafficking and distribution of DSCs. We used digital subtraction angiography, computed tomography and hematoxylin-eosin stainings to determine biodistribution of cells and to assess safety end-points. We found that selective injection of human DSCs to the rabbit SMA does not result in acute embolic complications. Furthermore, we found that i.v. administration result in extensive retention of the radiolabelled DSCs in the lungs, corroborating previous studies on pulmonary trapping. In shar...
Source: Cell Transplantation - Category: Cytology Authors: Tags: Cell Transplant Source Type: research