Identification of collaborative cross mouse strains permissive to Salmonella enterica serovar Typhi infection

Sci Rep. 2023 Jan 9;13(1):393. doi: 10.1038/s41598-023-27400-1.ABSTRACTSalmonella enterica serovar Typhi is the causative agent of typhoid fever restricted to humans and does not replicate in commonly used inbred mice. Genetic variation in humans is far greater and more complex than that in a single inbred strain of mice. The Collaborative Cross (CC) is a large panel of recombinant inbred strains which has a wider range of genetic diversity than laboratory inbred mouse strains. We found that the CC003/Unc and CC053/Unc strains are permissive to intraperitoneal but not oral route of S. Typhi infection and show histopathological changes characteristic of human typhoid. These CC strains are immunocompetent, and immunization induces antigen-specific responses that can kill S. Typhi in vitro and control S. Typhi in vivo. Our results indicate that CC003/Unc and CC053/Unc strains can help identify the genetic basis for typhoid susceptibility, S. Typhi virulence mechanism(s) in vivo, and serve as a preclinical mammalian model system to identify effective vaccines and therapeutics strategies.PMID:36624251 | DOI:10.1038/s41598-023-27400-1

Source: Cancer Control - January 9, 2023 Category: Cancer & Oncology Authors: Kishore R Alugupalli Sudeep Kothari Matthew P Cravens Justin A Walker Darren T Dougharty Gregory S Dickinson Louis A Gatto Andreas J B äumler Tamding Wangdi Darla R Miller Fernando Pardo-Manuel de Villena Linda D Siracusa Source Type: research