Molecular detection and characterization of Shigella spp. harboring extended-spectrum β-lactamase genes in children with diarrhea in northwest Iran

AbstractShigellosis is one of the acute bowel infections and remains a serious public health problem in resource-poor countries. The present study aimed to survey the distribution of extended-spectrum β-lactamase (ESBL)-producingShigella strains isolated from patients with diarrhea in northwest Iran. In the present cross-sectional study, from January 2019 to December 2020, 1280 fecal samples were collected from children with diarrhea in Ardabil, Iran. Multiplex PCR assay was applied for the presence ofipaH,invC,wbgZ,rfpB, andrfc genes to detectShigella spp.,Shigella sonnei,Shigella dysenteriae,Shigella flexneri, andShigella boydii, respectively. Phenotypic detection of ESBL-producing isolates was carried out using the Double Disc Test (DDT). The frequency of main ESBL encoding genes includingblaCTX-M,blaSHV, andblaTEM was detected using multiplex PCR. The genetic similarity ofS. sonnei isolates was determined using ERIC PCR. A total of 49Shigella isolates (3.8%; 49/1280) including 42 (85.7%)S. sonnei, 5 (10.2%)S. flexneri, and 2 (4%)S. dysenteriae were identified.S. boydii was not detected in any fecal samples. ESBLs were produced by 10.2% ofShigella spp. including 3S. sonnei, 1S. flexneri, and 1S. dysenteriae. The ESBL encoding genes includeblaCTX-M andblaTEM found in 65.3% and 61.2% of isolates, respectively.blaSHV gene was not detected in any isolates. The ERIC-PCR profiles allowed the differentiation of 42S. sonnei strains into 6 clusters. Our study revealed a high frequ...
Source: Molecular and Cellular Pediatrics - Category: Cytology Source Type: research