Molecules, Vol. 27, Pages 8094: Neuropeptides, New Ligands of SARS-CoV-2 Nucleoprotein, a Potential Link between Replication, Inflammation and Neurotransmission
Molecules, Vol. 27, Pages 8094: Neuropeptides, New Ligands of SARS-CoV-2 Nucleoprotein, a Potential Link between Replication, Inflammation and Neurotransmission
Molecules doi: 10.3390/molecules27228094
Authors:
Julien Henri
Laetitia Minder
Kevin Mohanasundaram
Sébastien Dilly
Anne Goupil-Lamy
Carmelo Di Primo
Anny Slama Schwok
This work identifies new ligands of the nucleoprotein N of SARS-CoV-2 by in silico screening, which used a new model of N, built from an Alphafold model refined by molecular dynamic simulations. The ligands were neuropeptides, such as substance P (1-7) and enkephalin, bound at a large site of the C-terminal or associated with the N-terminal β−sheet. The BA4 and BA5 Omicron variants of N also exhibited a large site as in wt N, and an increased flexibility of the BA5 variant, enabling substance P binding. The binding sites of some ligands deduced from modeling in wt N were assessed by mutation studies in surface plasmon resonance experiments. Dynamic light scattering showed that the ligands impeded RNA binding to N, which likely inhibited replication. We suggest that the physiological role of these neuropeptides in neurotransmission, pain and vasodilation for cholecystokinin and substance P could be altered by binding to N. We speculate that N may link between viral replication and multiple pathways leading to long COVID-19 symptoms. Therefore, N may constitute a “danger hub” that...
Source: Molecules - Category: Chemistry Authors: Julien Henri Laetitia Minder Kevin Mohanasundaram S ébastien Dilly Anne Goupil-Lamy Carmelo Di Primo Anny Slama Schwok Tags: Article Source Type: research