Effects of hydroxyapatite nanorods prepared through Elaeagnus Angustifolia extract on modulating immunomodulatory/dentin –pulp regeneration genes in DPSCs

This study determined the impacts of nano-hydroxyapatite (nHA) prepared throughElaeagnus Angustifolia extract (EAE) to enhance the relative expression of immunomodulatory/dentin –pulp regeneration genes in DPSCs. To produce nHA and modified nHA via EAE (nHAEA), the sol–gel technique was used. The functional groups of nanoparticles (NPs), morphological, and optical features were determined using Fourier transform infrared (FTIR), X-ray diffraction (XRD), Scanning electro n microscopy (SEM) together with energy-dispersive X-ray analysis (EDAX), and Transmission electron microscopy (TEM). The cell viability was then determined using the MTT method in the presence of various EAE, nHA, and nHAEA concentrations. Target gene expression was quantified using a real-time PCR procedure after treating DPSCs with an optimally non-toxic dose of EAE and NPs. The presence of the HA phase was reported with the XRD and FTIR results. According to the results of SEM and TEM, the rod-like NPs could be fabricated. nHAEAs were found to be characterized with low crystallite size, re duced diameter, lengthier, needle-like, and less agglomerated particles compared with nHA. The real-time PCR results demonstrated that nHAEA remarkably increased the expression of human leukocyte antigen-G5 (HLA-G5), vascular endothelial growth factor (VEGF), dentin sialophosphoprotein (DSPP), and i nterleukin6 (IL6) genes compared to the nHA group. These findings suggest that nHAEAs might have the potential applicat...
Source: Odontology - Category: Dentistry Source Type: research